Sticky ends from different BspQI sites may not be compatible. Sticky ends from different BstAPI sites may not be compatible.Įfficient cleavage requires at least two copies of the NarI recognition sequence.Įfficient cleavage requires at least two copies of the PluTI recognition sequence. G C A N N N N N T G C C G T N N N N N A C G Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different PfoI sites may not be compatible. Sticky ends from different AlwNI sites may not be compatible. Sticky ends from different AhdI sites may not be compatible.
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